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KMID : 0615720010180020101
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Jouranl of Korean Acupuncture and Moxibustion society
2001 Volume.18 No. 2 p.101 ~ p.110
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Effect of Scutellaria baicalensis Georgi Aquacupuncture on Oxidant - induced Cell Injury in Renal Cortical Slices
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Abstract
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Objective : This study was undertaken to determine if Scutellaria baicalensis Georgi (SbG) extract exerts protective effect against oxidant-induced cell injury in renal proximal tubular cells.
Methods : The cell injury was evaluated by lactate dehydrogenase (LDH) release in rabbit renal cortical slices and lipid peroxidation was estimated by measuring malondialdehyde (MDA). t-Butylhydroperoxide (tBHP) was used as a model of oxidant.
Results : tBHP at 1 mM increased LDH release and lipid peroxidation, which were prevented by SbG in a dose dependent manner over concentration range of 0.001-0.1%. SbG provided the protective effect against oxidant-induced reduction in PAH uptake by renal cortical slices and microsomal Na+-K+-ATPase activity. SbG attenuated tBHP-induced depletion of reduced glutathione. 0.2 mM HgCl₂ increased LDH release and lipid peroxidation, which were completely prevented by 0.05% SbG.
Conclusion : SbG prevents oxidant-induced impairment in membrane transport function.
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KEYWORD
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